Sample preparation

SY Sho Yoshimura
HT Hisashi Tanaka
SK Shuichi Kawabata
JK Junji Kozawa
HT Hiroto Takahashi
YH Yoh Hidaka
MH Masaki Hotta
NK Nobuo Kashiwagi
NT Noriyuki Tomiyama
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A 50% glucose solution (Fuso Yakuhin Kogyo, Osaka, Japan) was diluted with physiological saline (Otsuka Pharmaceutical, Tokyo, Japan) to target concentrations of glucose solutions of 0, 50, 100, 200, 300, 500, and 700 mM. Then, the pH of the diluted glucose solutions was adjusted to pH 6 by adding dilutions of 0.1 mol/L hydrochloric acid (Kanto Chemical, Tokyo, Japan) and/or 0.01 mol/L sodium hydroxide (Kanto Chemical).

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