Sequence data were obtained from the non‐redundant sequence database accessible at the National Center for Biotechnology Information website (http://www.ncbi.nlm.nih.gov). The genome sequence of B. dentium ATCC 27678 (accession number: ABIX02000002.1) in combination with Artemis Informatics Software (Wellcome Trust Sanger Institute) was used for genome resource, display, and analysis. BLAST searches were performed using the National Center for Biotechnology Information website. Sequence analysis was performed using the VectorNTI software package. GadB 3D structure modeling was achieved by means of Phyre2 software (http://www.sbg.bio.ic.ac.uk/phyre2/html/page.cgi?id=index). Visualization file was generated with Molmol.46
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.