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2.12. Photomicrography and image analysis
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Distal colon sections were examined under LEICA research microscope (DM750) connected to a digital camera (LEICA ICC50 - HD), and permanent photomicrographs were taken at the Department of Anatomy and Cell Biology, Obafemi Awolowo University, Ile-Ife, Nigeria. Representative micrographs were uploaded to the Fiji image J analysis software, and scale was set using a digital micrometer gauge reading to convert measurements in pixels to microns. Nuclear DNA intensity was determined on Feulgen stained micrographs (medium power, 400x).
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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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