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Real-time PCR was performed according to the manufacturer's instructions. The primers of miR-1224-3p, miR-328 and miR-214 were ordered from GenePharma Company. All experiments were performed using biological triplicates and experimental duplicates. The relative expression was calculated via the 2-ΔΔCt method.
Copyright and License information: ©2016 Wang et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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