Whole-Brain Data Analysis

Before all stimulation, resting-state BOLD brain activity was obtained for each subject and ROI (vide infra). All comparisons were done against this measurement. Fifteen minutes after the resting-state scan, the animals received thermonociception (vide supra) and a second acquisition time was obtained at the same time (Thermo scan). Fifteen minutes after thermonociception, a third acquisition time was taken to see the state of activation of all the ROIs previous to sciatic denervation (Pre-denerv scan). Following this acquisition, the subjects were denervated with acquisition times at 0, 15, 30, 45, 60 and 75 minutes. A 1-sample t-test was performed for each subject at each acquisition time, to detect the voxels with higher signal intensity over the mean for the 11 volumes encompassing each acquisition.