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Rat subcutaneous model

SS Salwa Suliman
AM Anna Mieszkowska
JF Justyna Folkert
NR Neha Rana
SM Samih Mohamed-Ahmed
TF Tiziana Fuoco
AF Anna Finne-Wistrand
KD Kai Dirscherl
BJ Bodil Jørgensen
KM Kamal Mustafa
KG Katarzyna Gurzawska-Comis
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Two incisions (~ 2 cm) were made on the back of 6–8-week-old Wistar rats after being anesthetized using isoflurane (Isoba® vet) (Schering Plough, NJ, USA). A pouch was dissected on each side and the unfunctionalized PLCA scaffolds (Control) or functionalized PLCA scaffolds (PA) were implanted subcutaneously and randomly distributed among all rats (at least n = 5 rats per time point). Wounds were sutured with Vicryl 4-0, and the animals were given buprenorphine (Temgesic® 0.3 mg/kg) subcutaneously as analgesic. Animals were euthanized with CO2 overdose at 4 days (acute inflammatory response) and 4 weeks (chronic inflammatory response) after implantation. The samples were harvested and stored in RNAlater (Invitrogen, Carlsbad, CA, USA) at − 80 °C until processed.

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