Enrichment Analysis

Gene enrichment analysis of the screened drug targets and intersectional targets was performed to further elucidate the mechanisms underlying the potency of celastrol. Both KEGG signaling pathway enrichment and GO biological functional enrichment analyses were performed using Metascape (http://metascape.org/gp/index.html#/main/step1) (Zhou et al., 2019). All genes in the genome were used as the enrichment background. Terms with a p-value of < 0.01, a minimum count of 3, and an enrichment factor of > 1.5 were collected and grouped into clusters based on their membership similarities. Data visualization was performed using an online platform (http://www.bioinformatics.com.cn).