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Quantitative real‐time RT–PCR analysis

MM Masahito Mizuuchi
TC Tereza Cindrova‐Davies
MO Matts Olovsson
DC D Stephen Charnock‐Jones
GB Graham J Burton
HY Hong Wa Yung
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Total RNA was isolated using RNeasy Mini Kits (Qiagen, Manchester, UK), according to the manufacturer's instructions, and the quantitative PCR performed using SYBR Green JumpStart kits (Sigma, Dorset, UK). Details of the primer sequences (Table S1) and other procedures are described in Supplementary materials and methods (see supporting information). The PlGF primers detected three of the four isoforms of PlGF, including PlGF‐1, −2 and −3.

Copyright and License information:  ©2015 The Authors. published by John Wiley & Sons Ltd on behalf of Pathological Society of Great Britain and Ireland.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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