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cDNA library preparation and RNA-sequencing
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Total RNA was extracted from nine HCC and cirrhotic tissues. We performed whole transcriptome sequencing for mRNA and lncRNA, as described in our previous study [52]. All sequencing was carried out at the Beijing Genomic Institute at Shenzhen (BGI-Shenzhen, Shenzhen, China). A detail description of the library preparation and sequencing is provided in the Supplementary materials.
Copyright and License information: ©2016 Pan et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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