4.8. Body Temperature Measurement

Mice rectal temperature was assessed using a VT4810 controller (Vertex Technology Corp., New Taipei City, Taiwan) adapted with a mouse rectal probe. In the first experiment, the impact of anandamide (10 mg·kg⁻¹) on body temperature was recorded during 30 min on animals injected either 20 min earlier with vehicle or JM-00266 (20 mg·kg⁻¹). In a second experiment, mice were injected either with the vehicle, Rimonabant (10 mg·kg⁻¹) or JM-00266 (20 mg·kg⁻¹) 20 min before receiving the selective CB1R agonist ACEA (1 mg·kg⁻¹) and rectal temperature was recorded 30 min later.