2.5. Dynamic Light Scattering (DLS)

JW Jarosław Wawer
JK Jakub Karczewski
RA Robert Aranowski
RP Rafał Piątek
DA Danuta Augustin-Nowacka
PB Piotr Bruździak
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The K-peptide solutions with concentrations of 2 mg/mL were used for analysis. The samples were placed in disposable cuvettes with optical lengths of 1 cm. Dynamic light scattering was measured using Malvern Zetasizer Nano ZS. The wavelength of the laser was equal to 633 nm. The radiation was collected in the backscatter mode (angle 173°). Each scan was measured for 20 seconds and, for a single determination, 20 runs were accumulated. Three scans obtained in this way were averaged for each sample. Temperature of the compartment was equal to 25 °C. The standard set of parameters were used in data analysis. The values 1.450 and 0.001 were accepted as the refractive index of material and the absorption, respectively. For dispersant, a viscosity of 0.8872 cP and refractive index of 1.330 were taken. The particle size distributions were obtained using non-negative least squares method (NNLS). The correlation functions for dynamic light scattering measurements are shown in Supplementary Materials in Figure S1.

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