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The oral cancer cell lines were genotyped and authenticated as described45. The HuR isoforms tagged with GFP such as full-length HuR, HuR-D226A, HuR-CP1 (gift from Dr. Gallouzi laboratory, McGill University) have been previously described (27). The transfections of plasmids were conducted by using Lipofectamine-2000 (Life Technologies). For HuR transient silencing experiments, cells were transduced with either a scrambled shRNA or two different shRNAs targeting CDS and 3'UTR of HuR (Sigma clones: TRCN0000017274 and TRCN0000276186).
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