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To evaluate the safety of PTX-NaHCO3-PLGA NPs in vitro, we co-incubated different concentrations of NPs (0.1, 0.5, 1.0, 5.0, 10, and 15 mg/mL) with rat VSMCs at 37 °C overnight, and cell viability was assessed using the standard Cell Counting Kit (CCK-8; n = 6), according to the following equation:
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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