3.3. DPPH Scavenging Assay

The DPPH scavenging activity assay was performed according to the method reported by Alhaithloul et al. [38]. Aliquots (0.2 g) of the dried samples were extracted with 45 mL 70% methanol in a water bath at 70 °C for 60 min and centrifuged for 15 min at 4700× g. The supernatant was retained and used to determine DPPH scavenging activity. An aliquot (4 mL) of 2.0 × 10⁻⁴ mmol·L⁻¹ DPPH solution in 70% ethanol was added to 1 mL of the supernatant. The mixture was allowed to incubate for 30 min at room temperature in the dark, after which the absorbance at 517 nm was measured.