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TCID50 assays to determine viral titers were performed as described previously [36]. Briefly, MARC-145 cells were seeded in 96-well plates and then infected with serial 10-fold dilutions of supernatants in eight replicates. The plates were incubated for 72–96 h before virus titers were calculated. PRRSV titers are shown as the TCID50 per milliliter, calculated using the Reed–Muench method [37].
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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