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Cells were seeded 24 h before the start of the assay. The gel spot was removed according to the manufacturer’s instructions. Cells were washed with culture media, then 200 μL CAP or argon-treated medium was added. The assay was performed under low-serum conditions (CAL-78: 1% FCS, SW1353: 0.1% FCS). Cells were stained with staining solution after 24 h of incubation and imaged. Evaluation of the cell-free area was determined with ImageJ software, using the MRI wound-healing tool plug-in.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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