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Generation of Flp-In T-Rex Doxycycline-Inducible HeLa Cell Line.
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The Flp-In T-Rex doxycycline-inducible HeLa/HA-LDLR C646Y cell line was generated by Invitrogen Flip-in T-REx kit according to the manufacturer’s protocol. Briefly, pOG44 and pcDNA3.1/HA-LDLR vector were cotransfected into the cells and selected for by hygromycin B (200 μg/mL) and blasticidin (4 μg/mL). The successfully transfected cells were induced by 2 μg/mL doxycyclin.
Copyright and License information: ©2022 the Author(s). Published by PNAS.
This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY).
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