Cell proliferation assay

AN Ana Silvina Nacht
AP Andy Pohl
RZ Roser Zaurin
DS Daniel Soronellas
JQ Javier Quilez
PS Priyanka Sharma
RW Roni H Wright
MB Miguel Beato
GV Guillermo P Vicent
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T47DMTVL cells transfected with control or BRG1 and BRM siRNAs were cultured as described above. Cells (1 × 104) were plated in a 96‐well plate in the presence or absence of 10 nM R5020. The cell proliferation ELISA BrdU Colorimetric assay (Roche) was performed according to the manufacturer's instructions. Figure 4B shows the percentage increase of proliferation in the presence versus absence of R5020. The experiments were performed in quintuplicate.

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