Primers for the SOE PCR assay

JL Jungang Li
JO Jing Ouyang
JY Jing Yuan
TL Tongxin Li
ML Ming Luo
JW Jing Wang
YC Yaokai Chen
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Using the DNA sequence of the laboratory reference strain of M. tuberculosis (H37Rv) as a template, we designed PCR primers, the amplifying products of which would contain the mutation sites of the rpoB, embB, katG, and inhA promoters. These genes confer resistance to RFP, INH, and EMB. The specific sequences are shown in Table Table11.

Primer sequences for the SOE PCR assay

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