2.4. RNA extraction and cDNA synthesis

The mice were sacrificed at the last day of drug administration. Then, around 100 mg of lung was isolated from each mouse. The RNA was isolated from the mouse lungs using Trizol solution, as described by the instructions of the factory. After that, the isolated mRNA was converted to cDNA as following: 1 µg of total RNA was reconstituted in a reaction mixture containing 100 pmol oligo deoxythymine, 2.5 mM deoxy-nucleic triphosphate mixture, 0.1 M dithiothreitol, 1X reverse transcriptase buffer, and 10 units of reverse transcriptase isolated from Moloney Murine Leukemia Virus. This reaction mixture was incubated at 37°C for 50 minutes.