Lattice light-sheet microscopy

QY Qixin Yang
YM Yuchuan Miao
LC Leonard J Campanello
MH Matt J Hourwitz
BA Bedri Abubaker-Sharif
AB Abby L Bull
PD Peter N Devreotes
JF John T Fourkas
WL Wolfgang Losert
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The 3i lattice light-sheet microscope in the Johns Hopkins School of Medicine Microscope Facility was used for two-color, 3D imaging. Vegetative, single Dictyostelium cells were seeded on a circular 5 mm coverslip patterned with nanoridges, which was immersed in a bath of standard developing buffer throughout imaging.

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