Preparation of placental microsomes/cytosol and deiodinase/sulfotransferase activity assays

CL Christopher Leonetti
CB Craig M. Butt
KH Kate Hoffman
SH Stephanie C. Hammel
MM Marie Lynn Miranda
HS Heather M. Stapleton
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Preparation of placental microsomes and DIO3 assays were performed using a modified method from our laboratory [12, 30]. Preparation of placental cytosolic fractions and SULT assays were performed using a modified method from our laboratory [11]. A detailed set of protocols are provided in the Additional file 1: Supplemental material.

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