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HL Hien Thi Thu Le
AM Akshaya Murugesan
NC Nuno R. Candeias
TR Thiyagarajan Ramesh
OY Olli Yli-Harja
MK Meenakshisundaram Kandhavelu
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PCa cell lines, PC3 and DU145, were cultured in Minimum essential medium eagle (MEME; Sigma-Aldrich). Non-cancer cell lines HEK293 and MEF were maintained in Dulbecco’s modified eagle medium–high glucose (DMEM; Sigma-Aldrich). Mediums were supplemented with 10% Fetal bovine serum (Biowest, Nuaille, France), 0.1 mg/mL streptomycin, 100 U/mL penicillin (Sigma-Aldrich), and 0.025 mg/mL amphotericin B (Sigma-Aldrich). Cells were grown at 37 °C in a humidified condition of 5% CO2. Cells were passaged every 3–4 days using trypsin 1X (Sigma-Aldrich). To prepare cells for assays, cells were counted using trypan blue solution (Sigma-Aldrich) and countess II FL automated cell counter (ThermoFisher Scientific, Waltham, MA, USA).

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