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Total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen) according to the manufacturer’s instructions. The first strand of cDNA was synthesized using TransScript First-Strand cDNA Synthesis SuperMix (TransGen Biotech), and qRT-PCR was performed as described previously (Chen et al., 2014). The relative expression level of a target gene was normalized to that of rice ACTIN1. All primers used in qRT-PCR are listed in Supplemental Table S3.
Copyright and License information: ©2016 American Society of Plant Biologists. All Rights Reserved.
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