Bone mineral element analysis was evaluated by X-Ray Fluorescence (XRF). For XRF analysis, the tibia bones were inserted into the bone tube, then put in the proper place in equipment. The processed bones were then analyzed at 10–20 kV accelerating voltages by an XRF (PANalytical MiniPAL 4). All procedures were done at Physic and Central Laboratory, Faculty of Mathematic and Natural Science, University of Malang [22].
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