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Labeling of cellular DNA with propidium iodide was performed to determine cells in different stages of the cell cycle using Guava Cell Cycle Reagent (Guava Technologies) according to the manufacturer’s instructions. Cell cycle analysis was performed by fluorescence-activated cell sorter analysis (FACS) on a Guava easyCyte sorter (Millipore) using the Cytosoft software (Guava Technologies).
Copyright and License information: ©2017 The American Society of Gene and Cell Therapy.
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