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Quantitative RT-PCR

AC Ailsa J. Christiansen
LD Lothar C. Dieterich
IO Isabel Ohs
SB Samia B. Bachmann
RB Roberta Bianchi
SP Steven T. Proulx
MH Maija Hollmén
DA David Aebischer
MD Michael Detmar
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RNA was isolated as described in the Supplementary Methods. Gene expression was investigated by quantitative RT-PCR using FastStart Universal SYBR Green Master Mix (Roche), the 7900HT Fast Real-time PCR system (Life Technologies), and quantified using the 2−ΔΔCt method. Primers (Microsynth) are shown in Supplementary Methods Table 3. All data were normalized to the expression of the reference gene Rplp0.

Copyright and License information:  ©2016 Christiansen et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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