2.16. PAS (Periodic Acid Schiff) staining of glycogen content

Liver slices from fed or fasted WT and mGD mice were fixed in 10% formalin and included in paraffin. Then, paraffin sections were cut at 5 μm, deparaffinized and hydrated to water. Afterwards, slices were oxidized in 0.5% periodic acid solution for 5 min, rinsed in distilled water and placed in Schiff reagent for 15 min. At the end of incubation, slices were washed in tap water for 5 min, counterstained in Mayer's hematoxylin for 5 min and washed again in tap water for 5 min. Finally, slices were dehydrated and coverslip mounted using Canada balsam. The following day, the slices were observed using a DM 5000 B microscope Leica with a 20X objective.