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Cells were transfected with pcDNA3.1-TROP2 or with siRNA sequence. The supernatants were harvested 48 h later, and the levels of IL-6, IL-8, and IL-1β were quantified in the supernatants using an ELISA kit according to the manufacturer’s instructions (RD Biosciences). The ELISA assay results were obtained from three independent experiments performed in triplicate.
Copyright and License information: The Author(s). ©2016
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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