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hPMNs were isolated as described in reference 57, and cytotoxicity assays were performed as described previously (17, 57). Briefly, 2 × 105 hPMNs were added to a final volume of 100 µl/well of RPMI (Gibco) supplemented with 10 mM HEPES. Cells were intoxicated for 1 h at 37°C in 5% CO2. Ten microliters of CellTiter 96 Aqueous One Solution (CellTiter; Promega) was added, and the mixture was incubated at 37°C in 5% CO2 for 2 h. hPMN viability was assessed with a PerkinElmer EnVision 2103 Multilabel Reader.
Copyright and License information: ©2016 Balasubramanian et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
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