GraphPad Prism 6.04 (GraphPad Software, Inc.) was used for statistical analysis. Survival curves were calculated by using the Mantel-Cox log rank test. Vaccine efficacy was evaluated by one-way ANOVA with Tukey’s multiple-comparison test. Similarity of variances between groups compared was determined by the Brown-Forsythe test. For autophagy quantification, the Mann-Whitney test was used for pairwise comparison. Two-way ANOVA with Tukey’s multiple-comparison test was used to evaluate immunology data.
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