Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Preparation of cationic liposome
Ask a
question
Favorite
Liposome preparation was described previously.19 Briefly, the required amounts of DOTAP and DOPE were first dissolved in chloroform at the weight ratio of 1:1, and a thin lipid film was produced via slowly evaporating the chloroform overnight with a rotary evaporator under vacuum conditions. The lipid film was subsequently hydrated with double-distilled water at room temperature to produce a crude suspension of 1 mg/mL. The crude suspension was sonicated for 10 min to obtain the final product.
Copyright and License information: ©2016 Zhang et al. This work is published and licensed by Dove Medical Press Limited
The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
