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For testing ClbS protection from cell-free fluids

JS Justin E. Silpe
JW Joel W. H. Wong
SO Siân V. Owen
MB Michael Baym
EB Emily P. Balskus
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Overnight cultures of wild-type E. coli BW25113 harbouring either pTrc-clbS or pTrc-∆clbS were centrifuged (16,100g and 1 min) before addition at 10% v/v to co-cultures containing a 1:1 ratio of E. coli BW25113 harbouring the PR-lux reporter and E. coli BW25113 harbouring either BAC-pks or the empty BAC. Bioluminescence was measured after 24 h and quantified in a plate-reader as outlined below (see ‘E. coli-based reporter assay’).

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