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Cell viability was assessed by MTT [3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] assay. In brief, RF/6A cells were seeded into 96-well plates, and then transfected before incubating them with 100 µl of the sterile MTT labeling reagent (0.5 mg/ml; Sigma, M2128) at 37°C for 3 h. DMSO (150 μl/well, Sigma, W387520) was added into each hole and shook for 15 min in the dark. The absorbance (OD) at 450 nm was recorded and analyzed by a microplate reader (Molecular Devices).
Copyright and License information: ©2022 Shi, Han, Liu, Li, Lu, Jiang and Yao.
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