Transfection of TREM2 adenovirus in cardiomyocytes

The male C57BL6 mice (weight 20–22 g) have received thoracotomy after intraperitoneal pentobarbital (35 mg/kg) and are intubated. Mice have received 50 µL of intramyocardial injections of 5×10⁵ plaque-forming units (pfu) Ad.TREM2 and Ad.Null, respectively (grouping by random number method). The injection point was 1–2 mm above ligation point. After 7 days, the animals were euthanasia by over-dose of intraperitoneal pentobarbital (130 mg/kg) and GFP expression in cardiomyocytes was detected. Additionally, the myocardial tissue was devided into above injection, around injection and below injection according to injection point. The TREM2 expression in these three partes were determined using western blot (shown in Appendix 1).