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F-Tractin (the actin binding domain of rat Inositol trisphosphate 3-kinase A (lTPKA), residues 9–40, atgGGCATGGCGCGACCACGGGGCGCGGGGCCCTGCAGCCCCGGGTTGGAGCGGGCTCCGCGCCGGAGCGTCGGGGAGCTGCGCCTGCTCTTCGAA) (Spracklen et al., 2014; Johnson and Schell, 2009) was synthesized and inserted into pUASp by KpnI (5')/SpeI (3'). Myc-BicD was amplified from pAC-FRB-GFP-BicD (del Castillo et al., 2015) and inserted into pUASp vector by SpeI (5’)/XbaI (3’).
Copyright and License information: ©2022, Lu et al
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