Fecal and blood samples

JH Jingjuan Hu
FD Fan Deng
BZ Bingcheng Zhao
ZL Zebin Lin
QS Qishun Sun
XY Xiao Yang
MW Mei Wu
SQ Shida Qiu
YC Yu Chen
ZY Zhengzheng Yan
SL Sidan Luo
JZ Jin Zhao
WL Weifeng Liu
CL Cai Li
KL Ke Xuan Liu
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Blood samples were collected at preoperatively (T0) and at 0 h (T1), 2 h (T2), 6 h (T3), 12 h (T4), and 24 h (T5) after surgery in EDTA plasma tubes as well as in serum separator tubes for analyses of intestinal fatty acid-binding protein (IFABP) and citrulline respectively. Fecal samples were collected at preoperatively, and the relative abundance of L. murinus was quantified by real-time PCR. IFABP and citrulline in the plasma samples were measured at multiple time points, to allow for (T3-T0) concentration differences, respectively by means of a human IFABP ELISA Kit (Bio-Swamp, Wuhan, China) and Citrulline ELISA Kit (USCN, Wuhan, China), following the manufacturer’s instructions. The gastrointestinal complication score of the patient on the seventh day after surgery was performed according to the acute gastrointestinal injury (AGI) standard described previously [32]. The detection of L. murinus, IFABP, citrulline, and AGI scores were performed by researchers blinded to the group allocation.

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