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RNA was isolated (RNeasy Plus Mini Kit, 74134, Qiagen), and cDNA synthesized using iScript Reverse Transcription Supermix, Bio-Rad. qPCR was performed with FastStart Universal SYBR Green Master [Rox] (04913850001, Roche). Primer sequences are provided in the supplemental methods.
Copyright and License information: The Author(s) ©2016
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