Iso-Seq library preparation, PACBIO sequencing and analysis

WZ Wenping Zhang
QZ Qun Zhou
JL Jishan Lin
XM Xinyi Ma
FD Fei Dong
HY Hansong Yan
WZ Weimin Zhong
YL Yijing Lu
YY Yuan Yao
XS Xueting Shen
LH Lixian Huang
WZ Wanqi Zhang
RM Ray Ming
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The RNA of leaves, bracts and flowers were extracted separately, and then mixed equally according to total RNA amount of each sample for construct one SMRT cell library (0.5–10 kb). The samples were sent to Beijing Novegene Bioinformatics Technology Co., Ltd for library construct and Pacbio RSII sequence, and the library underwent SMRT sequencing using two SMRT cells. Subreads were filtered and subjected to CCS using the SMRT Analysis Server 2.2.0 (Pacific Biosciences of California, Inc). CD-hit (v4.6.8) [35] with identify 0.85 were used to filter redundancy isoforms.

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