The RNA of leaves, bracts and flowers were extracted separately, and then mixed equally according to total RNA amount of each sample for construct one SMRT cell library (0.5–10 kb). The samples were sent to Beijing Novegene Bioinformatics Technology Co., Ltd for library construct and Pacbio RSII sequence, and the library underwent SMRT sequencing using two SMRT cells. Subreads were filtered and subjected to CCS using the SMRT Analysis Server 2.2.0 (Pacific Biosciences of California, Inc). CD-hit (v4.6.8) [35] with identify 0.85 were used to filter redundancy isoforms.
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