2.4. Isolation of lymphocytes and peripheral blood mononuclear cells

Isolation of CD3⁺ T cells and CD19⁺ B cells from buffy coats from healthy blood donors, and peripheral blood mononuclear cells (PBMCs) from CLL patient samples, was performed as described [23, 24]. Isolated cells were cryopreserved in liquid nitrogen [24]. See Table 2 for patient characteristics.

Patient characteristics. B, bendamustine; C, cyclophosphamide; F, fludarabine; R, rituximab; f, female; m. male; M, mutated IGVH; n.e., not established; OFA, ofatumumab; UM, unmutated IGVH.

Samples collected

(if more than one)

Idelalisib

(with OFA at initiation)

Idelalisib

(with OFA at initiation)