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In vivo matrigel plug angiogenesis assay of mice

WD Wenbo Ding
XC Xiuwei Chen
LY Licheng Yang
YC Yaping Chen
JS Jie Song
WB Weiquan Bu
BF Bin Feng
MZ Meng Zhang
YL Yi Luo
XJ Xiaobin Jia
LF Liang Feng
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Angiogenesis in vivo was verified via the Matrigel plug assay. Matrigel (0.5 ml/plug) in liquid (BD Biosciences) contained VEGF (150 ng/ml) and then drugs were added: vehicle, sorafenib (15 mg/kg), SDC (2 mg/kg), or the combination of sorafenib and SDC. Matrigel mixes were injected subcutaneously in the mid-ventral abdominal region of each mouse. Mice were sacrificed and the plugs were completely excised and photographed. Matrigel was collected, the content of hemoglobin was measured using a hemoglobin assay kit (Sigma-Aldrich, St. Louis, MO, USA), according to manufacturer’s protocols.21,22

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