Reagents and Chemicals

All the primers (Table 1) used in this study were synthesized by Shanghai Sangon Biotech Company (Shanghai, China). Anti-FAM antibody, goat anti-mouse antibody, and nitrocellulose membrane were purchased from Zoonbio Biotechnology Company. Quencher and fluorescein double-labeled reporter molecule, biotin and quencher double-labeled reporter molecule, and crRNAs were bought from Shanghai Genebiogist Technology Co, Ltd (Shanghai, China). WarmStart LAMP Kit, LbaCas12a, NEB buffer 3.1, and WarmStart Bst 2.0 DNA Polymerase were obtained from New England Biolabs (NEB). Deoxynucleoside triphosphate (dNTPs) and Taq polymerase premixture were purchased from Takara (Beijing, China). Chlor-auric acid (HAuCl₄⋅3H₂O) and trisodium citrate were obtained from Sigma–Aldrich (Steinheim, Germany). Absorbent paper, glass fiber paper, and nitrocellulose film were purchased from Sartorius AG (Gottingen, Germany). Genomic and plasmid extraction kits were purchased from Qiagen GmbH (Hilden, Germany) and Tiangen Biotech (Beijing, China) Co., Ltd, respectively.

The Oligonucleotide Sequences Used as in This Study