Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
Mouse cells were isolated as described previously18. Briefly, flushed mouse lung tissue was minced and digested with 3 ml Collagenase A (catalog no. 10103586001, Roche; 1 mg ml−1 in PBS) in a 37 °C water bath for 1 h. Mixtures were titrated with no. 18-G needles and then pipetted through a 40-μm disposable cell strainer. After centrifuging at 300g for 5 min and washing with PBS, isolated cells were treated with red blood cell lysis buffer (catalog no. 00-4300-54; eBioscience) for 5 min on ice to lyse red blood cells.
Copyright and License information: The Author(s) ©2022
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
