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Macrophage phagocytosis

AK Alexander S. Kim
EW Evan C. Werlin
HK Hideo Kagaya
MC Mian Chen
BW Bian Wu
GM Giorgio Mottola
MJ Masood Jan
MC Michael S. Conte
MB Michael Bader
MB Michael Bader
MB Michael Bader
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Stimulation of phagocytosis activity is a characteristic pro-resolving effect of SPM. Murine Raw 264.7 cells were plated on 96-well plates at a density of 5x10^5 cells/ml. Cells were pre-treated with either no treatment, LPS (100ug/ml), 17R-RvD1 (100-1000nM) and benzo-RvD1 (100-1000nM) for 1 hour, then either S. aureus (50:1 particle to macrophage ratio) or Zymosan (10:1 particle to macrophage ratio) bioparticles labeled with pHrodo (Invitrogen) were added for phagocytosis. Plates were analyzed using a microplate reader (SpectraMax M2; Molecular Devices) to detect the intensity of bioparticle fluorescence.

Copyright and License information:  ©2022 Kim et al ©2022 Michael BaderThis is an open access article distributed under the terms of the , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ©2022 Michael BaderThis is an open access article distributed under the terms of the , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ©2022 Michael BaderThis is an open access article distributed under the terms of the , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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