4.4. Optimizing the Hygromycin B Concentration for the Selection of Transformants

For the optimization of the hygromycin B concentration in shoot induction selection, the callus was placed on optimal SIM that contained timentin (200 mg L⁻¹) and different concentrations of hygromycin B (0, 0.5, 1.0, 1.5, 2.0 or 2.5 mg L⁻¹). After 5 weeks of culture in a growth chamber (25 ± 1 °C, 16/8 h light/dark), shoot induction was investigated. All experiments were performed with three independent replicates, and each replicate contained 30 calli.