2.2. Secondary and Tertiary Structure Prediction of PEPf

AJ Ana Paula Junqueira-Kipnis
CS Carine de Castro Souza
AC Ana Carolina de Oliveira Carvalho
FO Fabio Muniz de Oliveira
VA Vinnycius Pereira Almeida
AP Alisson Rodrigues de Paula
MC Mara Rubia Celes
AK André Kipnis
ask Ask a question
Favorite

The secondary and tertiary structures of PEPf were generated using RaptorX (http://raptorx.uchicago.edu/ (accessed on 11 February 2022) [24]. The generated PDB file was refined using Galaxy Refine (http://galaxy.seoklab.org/ (accessed on 11 February 20221) [25]. The server generated five refined models, which were verified regarding the ψ and ϕ angles of their amino acids using Ramachandran plots. The structure with the lowest number of outliers was selected for representation.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A