The secondary and tertiary structures of PEPf were generated using RaptorX (http://raptorx.uchicago.edu/ (accessed on 11 February 2022) [24]. The generated PDB file was refined using Galaxy Refine (http://galaxy.seoklab.org/ (accessed on 11 February 20221) [25]. The server generated five refined models, which were verified regarding the ψ and ϕ angles of their amino acids using Ramachandran plots. The structure with the lowest number of outliers was selected for representation.
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