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3.8. Fractional Inhibitory Concentration (FIC) Index Assay

PH Prakash Kishore Hazam
CC Chin-Cheng Cheng
CH Chu-Yi Hsieh
WL Wen-Chun Lin
PH Po-Hsien Hsu
TC Te-Li Chen
YL Yi-Tzu Lee
JC Jyh-Yih Chen
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A 2-fold dilution of peptide in 50 µL of media was performed along the vertical axis of a 96-well plate. Similarly, 50 µL of the next drug was diluted 2-fold along the horizontal axis. Next, 50 µL of 106 cells was added, and the standard MIC protocol was performed [22]. The FIC index was calculated according to the following formula:

where FIC A is MIC A & B combined/MIC A and FIC B is MIC A & B combined/MIC A.

Copyright and License information:  ©2022 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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