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Mouse colon cancer cell line CT26.WT

RO Rocío Olivera-Salazar
MG Mariano García-Arranz
AS Aránzazu Sánchez
SO Susana Olmedillas-López
LV Luz Vega-Clemente
LS Luis Javier Serrano
BH Blanca Herrera
DG Damián García-Olmo
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This ATCC cell line was kindly provided by Miguel Urioste Azcorra from Spanish National Cancer Research Centre (CNIO), Madrid, Spain. CT26.WT was chosen because it has the KrasG12D mutation. The cells were maintained with Dulbecco’s Modified Eagle Medium (DMEM). Cells were harvested at 80%–90% confluence using Tripsin–EDTA and replated 0.5–1 × 104 cells/cm2 with two changes of media per week until use for the experiments.

OCs lines and CT26.WT cells were cultured with 1% ZellShield (Minerva Biolabs, Berlin, Germany) and 10% Fetal Bovine Serum (FBS) and incubated at 37 °C, 5% CO2.

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