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2.26. Quantification of Apoptosis by Tunnel-Assay
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In Situ Cell Death Detection Kit, Fluorescein (Merck, Germany) was employed to detect and quantify apoptosis in day 17 worms grown on OP50 or LPJBC5. The procedure was followed as per the manufacturer’s instructions. One hundred worms per bacterial-treated group were used to detect fluorescence intensity, and each experiment was performed in triplicate.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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