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Two milliliters of saliva were collected using Oragene.Discover DNA collection kits (DNA Genotek Inc., Ottawa, ON, Canada). DNA purification was done according to the prepIT.L2P manual protocol provided by the manufacturer. DNA quality and quantity control were performed using standard Agarose gel electrophoresis, NanoDrop spectrophotometer (Thermo Scientific, Wilmington, DE, USA) and Qubit fluorometer (Promega, Madison, WI, USA).
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